Enzyme lab

Determining Properties of an Enzyme Purpose: Enzymes serve as proteins that influence the key out of a answer. Enzymes are long chains of amino acids with interlinking shapes. There a variety of enzymes, all(prenominal) with their own righteousness and job. The prompt site on an enzyme is specific to a single substrate, which usher out activate the energy in the enzyme. Without enzymes normal metabolic reactions would be sluggish. The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The tack of temperature and pH resulting be testinged and their influence on the enzyme recorded. If the environment is cold, consequently enzymes will not activate and if pH is game, then enzymes will not activate. This I believe because at low temperatures reactions operate to slow down and at high pH allthing will denature. Techniques: Enzyme activity was determined in sevensome test tubes to each one with a distin guishable combination of buffers, H202, extract, and dye. sort tubes were put in a mass spectrometer and their absorbance recorded. Absorbance was recorded at 20 second intervals for two full minutes. A temperature effect on enzymes was deliberate by exposing test tubes to four different temperature environments. (4C, elbow room temperature, 32C, and 48C).Seven test tubes were assembled exactly the same as the sample before.

over again the test tubes were placed in a spectrometer and their absorbance was measured at intervals of 20 seconds for two minutes. The effect of pH on an enzyme was tested in nine tes t tubes, each with a different combination o! f, buffer pH, H202, extract, and dye. Again the test tubes were placed in a spectrometer and their absorbance recorded every twenty seconds for a full two minutes. Results: Enzyme Activity Time (sec) underground 2 & 3 .5mL ExtractTube 4 & 5 1.0mL ExtractTube 6 & 7 2.0mL Extract 20.11.011.4 40.11.011.9 60.11.012 80.12.012 100.12.012 120.12.012 Temperature effect on Enzyme Time (sec)Tube 3 & 4 4C...If you want to number a full essay, order it on our website: OrderCustomPaper.com

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